Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease
Article Title: Netrin‐1 Preserves Blood‐Brain Barrier Integrity Through Deleted in Colorectal Cancer/Focal Adhesion Kinase/RhoA Signaling Pathway Following Subarachnoid Hemorrhage in Rats
doi: 10.1161/JAHA.116.005198
Figure Lengend Snippet: Changes in expressions of downstream signaling pathway of Focal Adhesion Kinase ( FAK )/RhoA after exogenous Netrin‐1 ( NTN ‐1) treatment and NTN ‐1 si RNA pretreatment at 24 hours post–subarachnoid hemorrhage ( SAH ). Exogenous NTN ‐1 significantly further enhanced the FAK phosphorylation (A), which resulted in the suppression of RhoA activity (B). While the endogenous NTN ‐1 was depleted by use of NTN ‐1 si RNA , the p‐ FAK level decreased (A), which augmented the RhoA activation (B). The RhoA activity was negatively related to the levels of endothelial tight junction proteins including ZO ‐1 (C) and Occludin (D) in the ipsilateral hemisphere. Relative densities of each protein have been normalized against the sham group. n=6 for each group. * P <0.05 vs sham, # P <0.05 vs vehicle, and & P <0.05 vs NTN ‐1 and Scr si RNA . Scr si RNA indicates scramble si RNA ; siRNA, small interfering RNA.
Article Snippet: Following the manufacturer's instructions, rat NTN‐1 siRNA (Thermo Fisher Scientific, Waltham, MA), DCC siRNA (OriGene Technologies, Rockville, MD), or negative control scramble siRNA (OriGene Technologies) was dissolved in transfection reagent (OriGene Technologies).
Techniques: Phospho-proteomics, Activity Assay, Activation Assay, Small Interfering RNA